1. komponenta

Lecture type	Total
Lectures	30
Seminar	15

* Load is given in academic hour (1 academic hour = 45 minutes)

COURSE CONTENT:
1. Introduction
1.1. Advantages and Disadvantages of Biocatalysts
1.2. Enzyme Properties and Nomenclature
2. Application of Biocatalysts According to the Type of Catalyzed Reaction
2.1. Hydrolytic Reactions
2.1.1. Mechanistic and Kinetic Aspects
2.1.2. Hydrolysis of the Amide Bond
2.1.3. Ester Hydrolysis
2.1.3.1. Esterases and Proteases
2.1.3.2. Lipases
2.1.4. Hydrolysis and Formation of Organic Phosphates
2.1.5. Hydrolysis of Epoxides
2.1.6. Hydrolysis of Nitriles
2.2. Reduction Reactions
2.2.1. Recycling of Cofactors
2.2.2. Reduction of Aldehydes and Ketones Using Isolated Enzymes
2.2.3. Reduction of Aldehydes and Ketones Using Whole Cells
2.3. Oxidation Reactions
2.3.1. Oxditation of Alcohols and Aldehydes
2.3.2. Hydroxylation of Alkanes
2.3.3. Hydroxylation of Aromatic Compounds
2.3.4. Epoxidation of Alkenes
2.3.5. Baeyer-Villiger Reactions
2.3.6. Formation of Peroxides
2.4. Formation of Carbon-Carbon Bonds
2.4.1. Aldol-Reactions
2.4.2. Acyloin-Reactions
2.4.3. Michael-Type Additions and Analogous Reactions
2.5. Addition and Elimination Reactions
2.5.1. Cyanohydrin Formation
2.5.2. Addition of Water and Ammonia
2.6. Glycosyl-Transfer Reactions
2.6.1. Glycosyl Transferases
2.6.2. Glycosidases
2.7. Halogenation and Dehalogenation Reactions
2.7.1. Halogenation
2.7.2. Dehalogenation
3. Special Techniques
3.1. Enzymes in Organic Solvents
3.1.1. Ester Synthesis
3.1.2. Lactone Synthesis
3.1.3. Amide Synthesis
3.1.4. Peptide Synthesis
3.1.5. Peracid Synthesis
3.1.6. Redox Reactions
3.1.7. Medium Engineering
3.2. Immobilization of Biocatalysts
3.3. Modified and Artificial Enzymes
3.3.1. Modified Enzymes
3.3.2. Semisynthetic Enzymes
3.3.3. Catalytic Antibodies


LEARNING OUTCOMES:
After completion of this course, the successful student will be able to:
- list the properties of enzymes as biocatalysts and to compare their advantages and disadvantages in regard to the chemical catalysts
- explain main principles of enzymatic catalysis
- classify enzymes according to the type of reaction and to predict what type of biocatalysts
should be used in particular (stereo)chemical transformations
- suggest techniques for optimization of selectivity of enzymatic reactions
- assess (stereo)chemical outcome of the biocatalytic reaction
- apply acquired knowledge of enzymatic catalysis in organic synthesis.

1. K. Faber: Biotransformations in Organic Chemistry, A Textbook, 6. izd., Springer-Verlag Berlin Heidelberg, 2011.
2. K. Drauz, H. Waldmann (ur.): Enzyme Catalysis in Organic Synthesis, 2. izd., Wiley-VCH, Weinheim, 2002.

Izborni predmeti izvan odabranih grana - Regular study - Analytical and inorganic chemistry
Izborni predmeti organska kemija - Regular study - Analytical and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Analytical and physical chemistry
Izborni predmeti izvan odabranih grana - Regular study - Analytical chemistry and biochemistry
Izborni predmeti organska kemija - Regular study - Biochemistry and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Biochemistry and physical chemistry
Izborni predmeti organska kemija - Regular study - Inorganic and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic and physical chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic chemistry and biochemistry
Izborni predmeti organska kemija - Regular study - Physical and organic chemistry

Izborni predmeti izvan odabranih grana - Regular study - Analytical and inorganic chemistry
Izborni predmeti organska kemija - Regular study - Analytical and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Analytical and physical chemistry
Izborni predmeti izvan odabranih grana - Regular study - Analytical chemistry and biochemistry
Izborni predmeti organska kemija - Regular study - Biochemistry and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Biochemistry and physical chemistry
Izborni predmeti organska kemija - Regular study - Inorganic and organic chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic and physical chemistry
Izborni predmeti izvan odabranih grana - Regular study - Inorganic chemistry and biochemistry
Izborni predmeti organska kemija - Regular study - Physical and organic chemistry