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Macromolecular Crystalography

Code: 152833
ECTS: 5.0
Lecturers in charge: prof. dr. sc. Dubravka Matković-Čalogović - Lectures
Take exam: Studomat

1. komponenta

Lecture typeTotal
Lectures 10
Seminar 5
* Load is given in academic hour (1 academic hour = 45 minutes)
Crystallization - sample preparation for X-ray diffraction experiments, determination of crystal symmetry. Data collection. Evaluation of X-ray diffraction intensities. Phase problem solution. Fourier synthesis. Interpretation of electron density maps, rough model. Refinement of the rough model and adjustment with measured intensities. Primary, secondary, tertiary structure of the macromolecule. Structural evaluation of the model and recognition of the protein biological function by using databases and structural bioinformatics (Cambridge Structural Database, Protein Data Bank, SwissProt, Phyre, etc.).
    Protein Crystallography, Methods and Protocols, Eds: A. Wlodawer, Z. Dauter, M. Jaskolski , Humana Press, 2017; DOI: 10.1007/978-1-4939-7000-1
    B. RUPP - Biomacromolecular crystallography: principles, practice and applications to structural biology. Abingdon, New York: Garland Science, Taylor & Francis Group, 2010.
    Li-Ling Ooi - Principles of X-ray Crystallography, , Oxford university press, 2010
    McPHERSON - Introduction to macromolecular crystallography, Wiley-Liss, 2003
    A. McPherson: Introduction to Macromolecular Crystallography, Wiley-Liss, Hoboken, New
    Jersey 2003
    D. Blow - Outline of crystallography for biologist, Oxford university press, 2002
    A.M. Lesk: Introduction to protein architecture, Oxford University Press, Oxford 2001.
    Practical Protein Crystallography, D.E. McRee: Practical Protein Crystallography, 2nd Edition, Academic Press, 1999.
    revijalni članci i znanstvene monografije
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