COURSE GOALS: The course should introduce students with elementary biochemical laboratory methods. Students use experimental methods to solve biochemical problems.
LEARNING OUTCOMES AT THE LEVEL OF THE PROGRAMME:
1. KNOWLEDGE AND UNDERSTANDING
1.2. demonstrate a thorough knowledge and understanding of the fundamental concepts in chemistry
1.4. demonstrate a thorough knowledge and understanding of the most important chemistry laws and theories
1.5. demonstrate knowledge and understanding of basic experimental methods, instruments and methods of experimental data processing in physics and chemistry
2. APPLYING KNOWLEDGE AND UNDERSTANDING
2.2. describe important aspects of chemical change
2.4. recognize and follow the logic of arguments, evaluate the adequacy of arguments and construct well supported arguments
2.6. prepare and perform classroom physics and chemistry experiments and interpret the results of observation
2.7. apply basic laboratory safety and security measures
2.9. use information and communication technology efficiently (to foster active enquiry, collaboration and interaction in the classroom)
4. COMMUNICATION SKILLS
4.1. communicate effectively with pupils and colleagues
4.2. present complex ideas clearly and concisely
5. LEARNING SKILLS
5.1. search for and use professional literature as well as any other sources of relevant information
LEARNING OUTCOMES SPECIFIC FOR THE COURSE:
1. to specify and explain basic rules for safe work in biochemical laboratory,
2. to independently perform experiments using standard biochemical techniques and methods,
3. to use basic biochemical equipment and instruments,
4. to appropriately handle biological macromolecules in order to avoid their denaturation and inactivation,
5. to specify and explain methods for purification and analysis of proteins and nucleic acids,
6. to perform enzyme kinetics experiments, including inhibition of enzyme reaction, and to determine kinetic parameters,
7. to apply mathematical methods for data analysis,
8. to graphically show results of experiments,
9. to critically evaluate experimental results,
10. to write a report on laboratory exercises.
Potentiometric titration of amino acids. Determination of alcohol-dehydrogenase kinetic parameters for ethanol. Inhibition of alcohol-dehydrogenase activity. Size-exclusion chromatography of biological macromolecules. Size-exclusion chromatography of hemoglobin. Polyacrylamide gel electrophoresis in the presence of SDS (SDS-PAGE). Electrophoresis of DNA on the agarose gel. Preparation of plasmid DNA from transformed bacterial cells.
REQUIREMENTS FOR STUDENTS:
Prior to the each practical exercise students's knowledge and understanding will be tested by quiz. The students who do not pass the quiz will not be able to perform that exercise. After successful finishing of the exercise students should write the report where they state the goal of the exercise, describe used methods and explain their results. Students will be encouraged to critically discuss obtained results.
GRADING AND ASSESSING THE WORK OF STUDENTS:
Students will be evaluated through the written exam.
- OBAVEZNA LITERATURA:
Interna skripta za Praktikum iz biokemije (13. radno izdanje), Zavod za biokemiju, PMF, Zagreb, 2007.
Relevantna poglavlja iz udžbenika L. Stryer, J. Berg i J. Tymoczko, BIOKEMIJA (6. izd), Školska knjiga, 2013. (prijevod na hrvatski jezik)
Relevantna poglavlja iz udžbenika J. M. Berg, J. L. Tymoczko i L. Stryer, BIOCHEMISTRY (7. izd.), W. H. Freeman & Co, New York, 2012.
- DOPUNSKA LITERATURA:
D. Voet i J. G. Voet, BIOCHEMISTRY (4. izd.), Wiley, New York, 2011.
D. L. Nelson i M. M. Cox, LEHNINGER PRINCIPLES OF BIOCHEMISTRY (6. izd.), W. H. Freeman & Co, New York, 2013.