Methods in Immunology


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Methods in Immunology

Code: 240548
ECTS: 6.0
Lecturers in charge: prof. dr. sc. Ana Galov
Lecturers: prof. dr. sc. Ana Galov - Seminar

prof. dr. sc. Ana Galov - Practicum
dr. sc. Gordan Kompes - Practicum
dr. sc. Blaženka Ladika Davidović - Practicum
Veronika Lončar , mag. biol. exp. - Practicum
dr. sc. Ida Svetličić - Practicum
dr. sc. Silvio Špičić - Practicum
dr. sc. Ivana Vrhovac Madunić - Practicum
Take exam: Studomat

1. komponenta

Lecture typeTotal
Lectures 30
Practicum 30
Seminar 15
* Load is given in academic hour (1 academic hour = 45 minutes)

It is expected that the students will:
-understand the fundamental scientific principles that underlie conventional and modern techniques in immunology
-gain practical experience in methods used in immunology
-be able to critically evaluate and asses methods available for addressing questions in immunology
-be competent to apply the knowledge gained during this course in a number of diverse fields


- Introduction and overview
- Antigens immunogens
- Antibodies - structure and function
- Antigen-antibody interactions - principles and applications
- Precipitation reactions, immunoelectrophoresis, agglutination reactions
- Coombs test, modifications of agglutination assays
- Radioimmunoassay, enzyme-linked immunosorbent assays
- ELISPOT, plaque-forming cell assay, immunoblotting, electrophoresis
- Immunoprecipitation, metabolic labeling, affinity chromatography, immunofluorescence, flow cytometry and fluorescence
- Alternatives to antigen-antibody reactions, immunoelectron microscopy
- Polyclonal and monoclonal antibodies - production and applications
- Experimental animal models in immunology
- Polymerase chain reaction, RT-PCR, sequencing, DNA cloning with plasmid vectors
- Immunogenetics - research of major histocompatibility complex genes

- flow cytometry immunophenotyping
- detection of antibodies by rapid slide agglutination, serotyping
- complement binding reaction
- enzyme-linked immunosorbent assay
- polymerase chain reaction
- agarose gel electrophoresis
- DNA cloning with plasmid vectors (ligation, transformation, transfer of selected colonies to culture medium, plasmid DNA isolation and purification)

Students are expected to prepare and present the topic from immunological methods or other biological methods used in immunology; or to present research article in which such methods are used.
  1. Andreis i sur. (2004): Imunologija, 6. izdanje
  2. Kindt TJ, Goldsby RA, Osborne BA (2007) Kuby Immunology. 6th ed. W.H. Freeman and company, New York
  3. Janeway CA, Travers P (1997) Immunobiology. The Immune System in Health and Disease 3rd ed. Current Biology , London.
  4. A. Ristov (glavna urednica) (2007) Metode u molekularnoj biologiji. Institut Ruđer Bošković, Zagreb
  5. Male D, Brostoff J, Roth DB, Roitt I (2006) Immmunology. 7th ed. Mosby Elsevier
  6. Day MJ, Schultz RD (2013) Veterinarska imunologija, Načela i primjena
1. semester
Mandatory course - Regular module - Physiology and Immunobiology
Consultations schedule: