1. komponenta

Lecture type	Total
Lectures	30
Practicum	30

* Load is given in academic hour (1 academic hour = 45 minutes)

COURSE AIM :
The aim of the course is to integrate knowledge from the fields of molecular genetics and human physiology, explain the mechanisms of development for different groups of diseases, and apply this knowledge in molecular diagnostics.
COURSE CONTENT:
LECTURES
1) Introduction to molecular diagnostics
2) Projects that led to the development of molecular diagnostics
3-4) Molecular diagnostic tests
5-6) Monogenetic diseases
7) Inherited syndromes
8-9) Genetics of malignant diseases
10) Metabolic syndrome and diabetes
11) Genetics of Alzheimer's and Parkinson's diseases
12) Pharmacogenetics
12-14) miRNA and cfDNA;
15) Molecular diagnostics in sports

LABORATORY PRACTICE
1) DNA isolation
2) RNA isolation and reverse transcription
3) PCR and multiplex PCR;
4) RT-qPCR
5) RFLP
6) Immunohistochemical algorithms
7-8) FISH
9-10) visit to clinical hospitals and analysis of the diagnostic process

1. Ambriović Ristov A i sur. Metode u molekularnoj biologiji. odabrana poglavlja
2. Revijalni i znanstveni članci, poglavlja iz prethodno navedenih knjiga predloženi od strane predavača na kolegiju
3. Barić I, Stavljenić-Rukavina A. Racionalna dijagnostika nasljednih i prirođenih bolesti. Medicinska naklada. odabrana poglavlja
4. Bruns DE, Ashwood ER, Burtis CA. Fundamentals of Molecular Diagnostics. Saunders, Elsevier. odabrana poglavlja
5. Nussbaum RL, McInnes RR, Willard HF. Genetics in medicine. Saunders, Elsevier. odabrana poglavlja

Izborni predmeti - Biologija čovjeka - Regular study - Molecular Biology