1. komponenta

Lecture type	Total
Practicum	30

* Load is given in academic hour (1 academic hour = 45 minutes)

COURSE CONTENT:
Potentiometric titration of amino acids. Determination of alcohol-dehydrogenase kinetic parameters for ethanol. Inhibition of alcohol-dehydrogenase activity. Size-exclusion chromatography of biological macromolecules. Size-exclusion chromatography of hemoglobin. Polyacrylamide gel electrophoresis in the presence of SDS (SDS-PAGE). Electrophoresis of DNA on the agarose gel. Preparation of plasmid DNA from transformed bacterial cells.

LEARNING OUTCOMES:
1. to specify and explain basic rules for safe work in biochemical laboratory
2. to independently perform experiments using standard biochemical techniques and methods
3. to use basic biochemical equipment and instruments
4. to appropriately handle biological macromolecules in order to avoid their denaturation and inactivation
5. to specify and explain methods for purification and analysis of proteins and nucleic acids
6. to perform enzyme kinetics experiments, including inhibition of enzyme reaction, and to determine kinetic parameters
7. to apply mathematical methods for data analysis
8. to graphically show results of experiments
9. to critically evaluate experimental results
10. to write a report on laboratory exercises

1. OBAVEZNA LITERATURA:
   Interna skripta za Praktikum iz biokemije (13. radno izdanje), Zavod za biokemiju, PMF, Zagreb, 2007.
   Relevantna poglavlja iz udžbenika L. Stryer, J. Berg i J. Tymoczko, BIOKEMIJA (6. izd), Školska knjiga, 2013. (prijevod na hrvatski jezik)
   ili
   Relevantna poglavlja iz udžbenika J. M. Berg, J. L. Tymoczko i L. Stryer, BIOCHEMISTRY (7. izd.), W. H. Freeman & Co, New York, 2012.
2. DOPUNSKA LITERATURA:
   D. Voet i J. G. Voet, BIOCHEMISTRY (4. izd.), Wiley, New York, 2011.
   D. L. Nelson i M. M. Cox, LEHNINGER PRINCIPLES OF BIOCHEMISTRY (6. izd.), W. H. Freeman & Co, New York, 2013.

Enrollment :
Enrolled : Biochemistry
Passed : Cell Biology

Mandatory course - Regular study - Biology and Chemistry Education