| Title: | Assistant Professor |
| Location: | -029 |
| Public phone number: | +385 1 4606 377 |
| Internal phone number: | 6377 |
| E-mail: | |
| Department: | Division of General and Inorganic Chemistry |
| Graduation year: | 2009 |
| PhD graduation year: | 2016 |
| Employed in this institution since: | 2011 |
graduate
integrated undergraduate and graduate
Education at Department of Chemistry, Faculty of Science, University of Zagreb: Diploma thesis (Biology and Chemistry, 2009); Doctoral thesis (in Chemistry, 2016).
Affiliation and current position: Division of General and Inorganic Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb: Assistant professor (2023–).
Visits to foreign research and education institutions: 2013 - 2014 PRIN 2010-2011 “Unraveling structural and functional determinants behind Helicobacter pylori pathogenesis and persistence”, Department of Biomedical Sciences, University of Padua. 2012 – PhD fellowship Croatian Science Foundation "Structural studies on the [NiFe]hydrogenase maturation system from Helicobacter pylori”, Department of Biomedical Sciences, University of Padua.
Research interest: Molecular cloning, protein expression and purification (affinity chromatography, size-exclusion chromatography), biophysical methods for protein characterization (UV-Vis spectroscopy and fluorimetry, CD, DLS), protein crystallization, protein and DNA X-ray crystallography and small molecule X-ray crystallography.
Scientific papers: https://www.croris.hr/crosbi/searchByContext/2/30194
Awards and recognitions and memberships: 2009 - Medal by the Department of Chemistry University of Zagreb for outstanding achievements throughout the studies, From 2011 - Member of the Croatian Chemical Society and the Croatian Crystallographic Association.
Disulfide bond enables cytosolic protein to function in oxidizing conditions
The Thirtieth Croatian-Slovenian Crystallographic Meeting
Crystallization of the Keap1-DPP III complex
Exploring the copper affinity toward the CrdA protein from Helicobacter pylori
Uloga disulfidne veze u biljnoj citosolnoj seril-tRNA-sintetazi
Evolutionarily conserved cysteines in plant cytosolic seryl-tRNA synthetase are important for its resistance to oxidation
Counter anion effects on the formation and structural transformations of mo(vi)-hydrazone coordination assemblies: salts, solvates, co- crystals, and neutral complexes
Zn(II) halide coordination compounds with imidazole and 2-methylimidazole. Structural and computational characterization of intermolecular interactions and disorder
Copper Binding and Oligomerization Studies of the Metal Resistance Determinant CrdA from Helicobacter pylori
Binding of peptide mimicking the ETGE loop of dipeptidyl peptidase III to the Kelch domain of Keap1
Contrasting effect of evolutionary conserved cysteines on the stability of plant seryl-tRNA synthetase
Emerging noncanonical functions of plant aminoacyl- tRNA synthetase
Binding of dipeptidyl peptidase III to the oxidative stress cell sensor Kelch-like ECH- associated protein 1 is a two-step process
Kristalizacija derivata inzulina
Comprehensive study on molybdenum(VI) complexes of 3-methoxysalicylaldehyde isonicotinoylhydrazone
Importance of the disulfide link in the stability of cytosolic protein
Influence of halide ions on crystallization and molecular and crystal structure of copper(II), nickel(II) and cobalt(II) derivatives of human insulin
Structural investigation of the role of the disulfide link in seryl-tRNA-synthetase from the plant Arabidopsis thaliana
Determination of interaction of copper ions and CrdA protein from Helicobacter pylori
Phenylboronic Acids Probing Molecular Recognition against Class A and Class C β-lactamases
Stability studies of selected Helicobacter pylori proteins required for survival
Plant seryl-tRNA synthetase as a link between translation and metabolism of brassinosteroid hormones
Plant seryl-tRNA synthetase as a link between translation and metabolism of brassinosteroid hormones
Arabidopsis seryl-tRNA synthetase: the first crystal structure and novel protein interactor of plant aminoacyl-tRNA synthetase
Revealing the structure of the first plant aminoacyl-tRNA synthetase at 2.3 Å
Pojačana ekspresija i kristalizacija proteina HP1026 iz bakterije Helicobacter pylori
Copper(II) complexes with N-alkyliminodiacetamide ligands. Preparation, structural, spectroscopic and DFT studies and biological evaluation
Overexpression, purification and crystallization of CrdA protein and optimization of crystallization of HP1026 protein from Helicobacter pylori
Where translation meets plant steroid metabolism: interaction of seryl-tRNA synthetase and BEN1 protein from Arabidopsis thaliana
Crystallization of putative copper binding CrdA protein and helicase-like HP1026 protein from the human pathogen Helicobacter pylori
Structural characterization of FlgE2 protein from Helicobacter pylori hook
Influence of the non-coordinating alkyl chain type in N-alkylated iminodiacetamides on the stability and structure of their complexes with nickel(II) and cobalt(II)
Biološki aktivni kompleksi bakra(II) s N-alkilnim derivatima iminodiacetamida
Crystal structure of truncated FlgD from the human pathogen Helicobacter pylori
Structure and Stability of FlgD from the Pathogenic 26695 Strain of Helicobacter pylori
Stability and structural characterization of the FlgD protein from Helicobacter pylori
Structural characterization of Heliobacter plyori proteinsrequired for survival of the bacterium
Reakcije niklovih(II) i kobaltovih(II) soli s derivatima iminodiacetamida
The first crystal structure of plant aminoacyl-tRNA synthetase: seryl-tRNA synthetase from Arabidopsis thaliana
Stability data of FlgD from Helicobacter pylori and structural comparison with other homologs
Kloniranje i ekspresija različitih varijanti HP1326 iz bakterije Helicobacter pylori
Crystal structure of truncated FlgD from the human pathogen Helicobacter pylori
Synthesis and structural studies of dioxomolybdenum(VI) complexes with isoniazid- related hydrazones
Kompleksi Co(II), Ni(II) i Cu(II) s N- cikloheksiliminodiacetamidom
Dioxidomolybdenum(VI) complexes with isoniazid- related hydrazones: solution-based, mechanochemical and UV-light assisted deprotonation†
Crystal Structure of Truncated FlgD from the Human Pathogen Helicobacter pylori
CHARACTERIZATION OF CrdA - THE COPPER HOMEOSTASIS FACTOR FROM BACTERIUM HELICOBACTER PYLORI
Structural Characterization at the Atomic Level of a Molecular Nano-Machine: The State of the Art of Helicobacter Pylori Flagellum Organization
Structural studies of FlgD - flagella hook capping protein from Helicobacter pylori
Structural and functional characterization of ATP binding HP1026 - Helicobacter pylori heat shock protein
Cloning, purification and crystallization of FlgD and CrdA – Helicobacter pylori survival required proteins
Spectroscopic and structural insights into N- substituted pyridinium-4-aldoximes and their pentacyanoferrate(II) complexes
Structural Characterization of the Metal Homeostasis Factors from Helicobacter Pylori
Crystallization of Insulin Derivatives
Structural studies about HypB and CrdA, two Helicobacter pylori metal homeostasis key factors
High Resolution Structure of the Manganese Derivative of Insulin
Kristalna struktura vanadijevog derivata inzulina
High resolution structure of the Mn derivative of insulin
Research methods in education (entrance exams in chemistry)
Studies on the reaction between hesperetin and titanium(IV) with spectrophotometric method
V. Ević, R. Šoić, Ruzica, M. Močibob, M. Kekez, J. Houser, M. Wimmerova, D. Matković-Čalogović, I. Gruić-Sovulj, I. Kekez, J. Rokov-Plavec. Evolutionarily conserved cysteines in plant cytosolic seryl-tRNA synthetase are important for its resistance to oxidation. FEBS Lett., 597 (23) (2023), 2975-2992.
I. Kekez, M. Faletar, M. Kekez, L. Cendron, M. Wright, G. Zanotti, D. Matković-Čalogović. Copper Binding and Oligomerization Studies of the Metal Resistance Determinant CrdA from Helicobacter pylori. Molecules, 27 (11) (2022), 3387.
S. Matić, I. Kekez, M. Tomin, F. Bogár, F. Šupljika, S. Kazazić, M. Hanić, S. Jha, H. Brkić, B. Bourgeois, M. Tobias, K. Gruber, P. Macheroux, D. Matković-Čalogović, M. Matovina, S. Tomić. Binding of dipeptidyl peptidase III to the oxidative stress cell sensor Kelch-like ECH-associated protein 1 is a two-step process. J. Biomol. Struct. Dyn. (2020)
P. Linciano, M. Vicario, I. Kekez, P. Bellio, G. Celenza, I. Martín-Blecua, J. Blázquez, L. Cendron, D. Tondi. Phenyl boronic acids probing molecular recognition against class A and class C β-lactamases. Antibiotics. 8 (4) (2019), 171.
M. Kekez, V. Zanki, I. Kekez, J. Baranašić, V. Hodnik, A.-M. Duchêne, G. Anderluh, I. Gruić Sovulj, D. Matković-Čalogović, I. Weygand- Đurašević, J. Rokov Plavec. Arabidopsis seryl-tRNA synthetase: the first crystal structure and novel protein interactor of plant aminoacyl-tRNA synthetase. FEBS J., 286 (3) (2019) 536-554.
V. Loconte, I. Kekez, D. Matković-Čalogović, G. Zanotti. Structural characterization of FlgE2 protein from Helicobacter pylori hook. FEBS J., 284 (24) (2017) 4328-4342.
I. Pulić, L. Cendron, M. Salamina, P. Polverino de Laureto, D. Matković-Čalogović, G. Zanotti. Crystal structure of truncated FlgD from the human pathogen Helicobacter pylori. J. Struct. Biol. 194 (2) (2016) 147-155.
Project Associate:
Development Research Grants, National Recovery and Resilience Plan, Next Generation EU: “Enzyme Engineering for Sustainable Recycling of Bioplastics”, NPOO.C3.2.R2-I1.06 (2023–2026);
Croatian Science Foundation Republic of Croatia project: “Biological relevance of dipeptidyl peptidase III and its impact on human health", HRZZ- IP-2018-01-2936 (2019-2023);
Croatian Science Foundation Republic of Croatia project: “Essential metal ions in H. pylori proteins and model complexes- structure and function/property” HRZZ-IP-2014-09-4274 (2015-2019);
Croatian Academy of Sciences and Arts project: "Crystallization and structural investigation of human insuline derivative and HP1026 protein from the Helicobacter pylori bacterium" (2016-2017);
PRIN 2010-2011, Republic of Italy project: “Unraveling structural and functional determinants behind Helicobacter pylori pathogenesis and persistence” (2013-2014);
Croatian Science Foundation, Republic of Croatia project: "Structural studies on the [NiFe]hydrogenase maturation system from Helicobacter pylori” (2012);
Ministry of Science, Republic of Croatia project: “Structural studies on biological macromolecules by X-ray diffraction” (2011-2013).
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