1. komponenta

Lecture type	Total
Lectures	30
Seminar	15

* Load is given in academic hour (1 academic hour = 45 minutes)

COURSE DESCRIPTION
The topic of the course is the introduction to model organisms through great discoveries in molecular biology.
THE AIM OF THE COURSE
The aim of the course is to teach about the biology of the model organisms and linked them to great discoveries in molecular biology.
COURSE CONTENT:
LECTURES
1. INTRODUCTION TO MODEL ORGANISMS; definition of a model organism, their use, general characteristics of model organisms, short overview of model organisms.
2. Escherichia coli AS A MODEL ORGANISM; survey of the biology and genome of E. coli, pathogenic strains, processes of conjugation, transformation and transduction, E. coli in genetics and biochemistry, new techniques
3. YEAST Saccharomyces cerevisiae; characteristics of yeast, mating types and their switch; discoveries on cell cycle, yeast artificial chromosomes, microarray techniques and two hybrid assay
4. SLIME MOLD Dyctiostelium; life cycle, chemotaxis and migration, cell differentiation, model in cell signalling
5. NEMATODE Caenorhabditis elegans; biology, discovery of apoptosis and gene silencing
6. FRUIT FLY Drosophila melanogaster; characteristics, establishment of chromosomal theory, transposons and jumping genes, P element and mutagenesis
7. Drosophila AS A MODEL IN DEVELOPMENTAL BIOLOGY; regulation of early embryonic development
8. FISH Danio rerio AS A VERTEBRATE MODEL; basic characteristics, zebra fish in embryology studies, as a model for human diseases and in toxicology studies; morpholino sequences as a tool for reverse genetic studies
9. FROG Xenopus, positive and negative characteristics as model organism, experiments leading to the discovery of embryonic development principles and cell cycle mechanism, lump brush chromosomes, transgenic animals
10. MOUSE AS A MODEL IN BIOMEDICINES AND HUMAN DISEASE INVESTIGATIONS; mice breeding and their characteristics, production of transgenic mice; isolation of embryonic stem cells
11. ANIMAL CELL CULTURE; basic principles of culturing cells in vitro, types of investigations done on cell culture, investigations on cytotoxicity, tumorigenesis, senescence, apoptosis, differentiation
12. Arabidopsis thaliana AS A MODEL PLANT; principles of early embryonal development in plants, production of transgenic plants
13. PLANT CELL CULTURE; basic conditions of plant cell culture, different types of plant cultures, use of plant cultures in biotechnology, production of recombinant plants
14-15. OTHER MODEL ORGANISMS; Axolotl, moss, sea urchin, Tardigrada
SEMINARS:
Each seminar is a presentation of a discovery in molecular biology as a consequence of an experimental work on model organism, according to scientific article by choice.

1. Molecular biology of the cell, Alberts B, Johnson A, Lewis J, Morgan D, Raff M, Roberts K, Walter P, Garland Science, Taylor and Fancis Group, New York, SAD, 2015.
2. The cell: a molecular approach, Cooper G. M, ASM Press Washington DC, Sinauer Ass. Sunderland Massachusetts, SAD, 2000.
3. Genetics, from genes to genomes, Hartwell LH, Hood L, Goldberg ML, Reynolds AE, Silver LM, Veres RC, McGrawHill, 2004.
4. Developmental biology, SF. Gilbert, Sinauer Associates Inc. Publichers Sunderland, Massachusetts, SAD, 2003.

Izborni kolegiji - Regular study - Molecular Biology

Izborni kolegiji - Regular study - Biology