* Load is given in academic hour (1 academic hour = 45 minutes)
1. analyse the structure of photosynthetic membranes and molecular complexes involved in photosynthesis
2. analyze the function of components of the photosynthetic apparatus
3. analyze the regulatory mechanisms involved in the process of photosynthesis and their dependence on endogenous and environmental signals
4. analyze the relationship between photosynthesis and other cellular processes and cellular compartments
5. use measurement instruments/techniques used in photosynthesis research
6. create protocols for the implementation of these methods/techniques
7. conduct scientific research using some of the methods/techniques for photosynthesis research
Through the latest knowledge in the field of photosynthesis research the course teaches students about the function of components of photosynthetic apparatus in relation to structure, as well as regulatory mechanisms and their response to endogenous and external signals
1.-3. Molecular organization of thylakoid membranes: photosynthetic pigments, photosystems, electron transfer chain.
4.-5. Regulations in the process of photosynthesis.
6. Regulation of nuclear gene transcription by chloroplast signals.
7. Import of proteins into the chloroplast.
8- 13. Photosynthesis under stress conditions: photoinhibition, influence of temperature, lack of water, increased salinity, presence of heavy metals and herbicides.
14. - 15. Evolution of the photosystem.
1.-4. Theory and discussion of methods and protocols used in the research of photosynthesis based on recent scientific papers. Students work on a given topic and present it as a 15-minute lecture.
5. Basic operation principle of a measurement instruments used in photosynthesis research.
6.-7. Students independently set up an experiment to investigate the effect of the stress factor on the process of photosynthesis
8.-9. Preparation of plant material and measurement of chlorophyll fluorescence by saturation pulse method and OJIP
10.-11. Extraction and separation of photosynthetic pigments by high-performance liquid chromatography
12.-13. Extraction of proteins and their separation by electrophoresis and immunochemical detection of some proteins involved in photosynthesis
14.-15. Statistical analysis and interpretation of the results.
- Buchanan, B., Gruissem, W., Jones, R. L.: Biochemistry and Molecular Biology of Plants, 2nd Edition
- Taiz, L., Zeiger, E.: Plant Physiology, Fifth Edition
- Nobel, P.: Physicochemical and Environmental Plant Physiology